Serotypes of sex pili

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Sex pili mutants isolated by macarbomycin treatment.

Macarbomycin (MM) preferentially kills Escherichia coli carrying episomes derepressed for sex pili formation (drd episomes). Using MM, we have isolated various types of mutants from both F-lac.tet and R100-1 episomes and have classified them into two types by sex pili formation. Among 26 mutants, 20 could not produce pili and had lost both the ability for conjugal transmission and sensitivity t...

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Serotypes of attachment pili of enterotoxigenic Escherichia coli isolated from humans.

Pili from enterotoxigenic Escherichia coli isolated from humans have been partially purified, and antisera have been prepared. These pili were initially attached to erythrocytes and then removed by thermal elution for purification. Three distinct antigenic types of pili have been identified. Antisera against these three pili types reacted with 60 of 106 (56%) enterotoxigenic E. coli isolated fr...

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Studies on sex pili: mutants of the sex factor F in Escherichia coli defective in bacteriophage-adsorbing function of F pili.

Ultraviolet irradiation or nitrosoguanidine treatment of Escherichia coli K-12 strain JE3100 (F'(8)/fla pil) led to the isolation of six mutants defective in F pili function. The defects were shown to be caused by mutations in the F factor. The mutants retained conjugal fertility, although they were less efficient than parental F'(8) strain, and continued to synthesize F pili. Three of the muta...

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Relationship between sex-pili formation and macarbomycin sensitivity in Escherichia coli.

The presence in Escherichia coli strains of derepressed sex factors determining F-type pili formation was found to increase their sensitivity to macarbomycin.

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Expression of Recombinant Protein B Subunit Pili from Vibrio Cholera

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...

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ژورنال

عنوان ژورنال: Epidemiology and Infection

سال: 1970

ISSN: 0950-2688,1469-4409

DOI: 10.1017/s0022172400042625